The Mass Spectrometry Research Incubator (VT-MSI) is an open facility that supports Virginia Tech's research infrastructure as a mass spectrometry analysis and education center. The facility provides the tools and skills needed for answering key questions in chemical and life sciences research.
The VT-MSI is designed to serve both funded research and proposal development efforts. As a collaborative research "incubator" we seek to develop and expand mass spectrometry-based proteomic and metabolomic efforts on the VT campus. We provide training in the areas of sample preparation, instrument operation and data analysis, with strong emphasis on the metadata and analytical requirements for publication of metabolomic and proteomic results.
Funding for the facility operations is provided in large part by the Department of Biochemistry and the Fralin Life Science Institute. Other key contributors include ICTAS and the Departments of Animal and Poultry Sciences, Biological Sciences, Chemistry, Human Nutrition Foods and Exercise, and Plant Pathology and Weed Science.
Those interested in proteomic or metabolomic work are strongly encouraged to contact the VT-MSI before embarking on proposal development, experimental design and/or sample collection.
Chen Y, Ray WK, Helm RF, Melville SB, Popham DL. Levels of Germination Proteins in Bacillus subtilis Dormant, Superdormant, and Germinating Spores. PLoS One. 9  e95781. doi: 10.1371/journal.pone.0095781. PubMed PMID: 24752279.
Ho TD, Williams KB, Chen Y, Helm RF, Popham DL, Ellermeier CD. Clostridium difficile Extra-Cytoplasmic Function s factor sV regulates lysozyme resistance and is necessary for pathogenesis in the hamster model of infection. Infect Immun. 2014 (in press). PubMed PMID: 24664503.
Webb BA, Hildreth S, Helm RF, Scharf BE. The Sinorhizobium meliloti chemoreceptor McpU mediates chemotaxis towards host plant exudates through direct proline sensing. Appl Environ Microbiol. 2014 (in press). PubMed PMID: 24657863.
Vilhauer L, Jervis J, Ray WK, Helm RF. The exo-proteome and exo-metabolome of Nostoc punctiforme (Cyanobacteria) in the presence and absence of nitrate. Arch Microbiol. 196  357-367. doi: 10.1007/s00203-014-0974-2. PubMed PMID: 24643449.
Arriola Apelo SI, Singer LM, Ray WK, Helm RF, Lin XY, McGilliard ML, St-Pierre NR, Hanigan MD. Casein synthesis is independently and additively related to individual essential amino acid supply. J Dairy Sci. 97  2998-3005. doi: 10.3168/jds.2013-7422. PubMed PMID: 24582441.
Zhang J, Bowles FL, Bearden DW, Ray WK, Fuhrer T, Ye Y, Dixon C, Harich K, Helm RF, Olmstead MM, Balch AL, Dorn HC. A missing link in the transformation from asymmetric to symmetric metallofullerene cages implies a top-down fullerene formation mechanism. Nat Chem. 5  880-5. doi: 10.1038/nchem.1748. PubMed PMID: 24056346.